PICALM Antibody (Center) [APR12866G]

PICALM Antibody (Center) [APR12866G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M, Rat

Specificity: This PICALM antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 385-415 amino acids from the Central region of human PICALM.

Cellular Localisation: Cell membrane. Membrane, clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle, clathrin- coated vesicle. Nucleus. Note=Colocalized with clathrin in the Golgi area (PubMed:10436022) . Interaction with PIMREG may target PICALM to the nucleus in some cells (PubMed:16491119)

Molecular Weight: 70755

Clone: Polyclonal

Gene Name: PICALM

Gene ID: 8301

Function: Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly (PubMed:10436022, PubMed:16262731, PubMed:27574975) . Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature (PubMed:25898166) . In addition to binding to clathrin, mediates the endocytosis of small R- SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8 (PubMed:22118466, PubMed:21808019, PubMed:23741335) . In turn, PICALM- dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors (PubMed:25241929) . Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP- CTF) (PubMed:25241929, PubMed:24067654) .

Summary: Tissue Location: Expressed in all tissues examined.

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:2000

Synonyms: Phosphatidylinositol-binding clathrin assembly protein, Clathrin assembly lymphoid myeloid leukemia protein, PICALM, CALM