Pig Lipoprotein lipase (LPL) ELISA Kit | AE63277PI

Pig Lipoprotein lipase (LPL) ELISA Kit | AE63277PI | Gentaur UK, US & Europe Distribution

Species Reactivity: Pig (Sus scrofa; Porcine)

Abbreviation: LPL

Alternative Name: HDLCQ11; LIPD;

Application: ELISA

Range: Request Information

Sensitivity: Request Information

Intra-Assay: ≤7.2%

Inter-Assay: ≤8.3%

Recovery: 1, 01

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate LPL in samples. An antibody specific for LPL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Lipoprotein lipase (EC 3.1.1.34) is an enzyme which hydrolyzes lipids in lipoproteins, like those found in chylomicrons and very low density lipoproteins (VLDL), into three free fatty acids and one glycerol molecule. It requires Apo-CII as a cofactor. Lipoprotein lipase is specifically found in endothelial cells lining the capillaries.Insulin is known to enhance LPL synthesis in adipocytes and its placement in the capillary endothelium. LPL has different isozymes in different tissues. The form that is in adipocytes is activated by insulin, whereas that in muscle and myocardium is not. This helps to explain why adipose cells gain fat in a well-fed state.A number sign is used with this entry because type I hyperlipoproteinemia can be caused by mutation in the lipoprotein lipase gene (LPL) .

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .