Pig TSH (Thyroid Stimulating Hormone) ELISA Kit | ELK8940

Pig TSH (Thyroid Stimulating Hormone) ELISA Kit | ELK8940 | Gentaur UK, US & Europe Distribution

Alternative Names: Thyrotropin

Species: Pig

Assay Type: Sandwich

Sensitivity: 0.11 uIU/mL

Standard: 12 uIU/mL

Detection Range: 0.19-12 uIU/mL

Sample Type: Serum

Assay Lenght: 3.5h

Research Area: Endocrinology;Hormone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyroid Stimulating Hormone (TSH) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyroid Stimulating Hormone (TSH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thyroid Stimulating Hormone (TSH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroid Stimulating Hormone (TSH) in the samples is then determined by comparing the OD of the samples to the standard curve.