PIK3R4 Antibody | 7949

PIK3R4 Antibody | 7949 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: PIK3R4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PIK3R4.
The immunogen is located within amino acids 1290 - 1340 of PIK3R4.

Research Area: Autophagy

Tested Application: E, WB, IHC-P, IF

Application: PIK3R4 antibody can be used for detection of PIK3R4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: PIK3R4 antibody is human, mouse and rat reactive.

Positive Control 1: Cat. No. 1315 - Human Tonsil Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 149 kDa
Observed: 140 kDa

Validation: N/A

Isoform: N/A

Purification: PIK3R4 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PIK3R4 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: PIK3R4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Phosphoinositide-3-kinase regulatory subunit 4, p150, Phosphoinositide 3-kinase adaptor protein, PI3-kinase p150 subunit, PI3-kinase regulatory subunit 4, VPS15

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: PIK3R4 (phosphoinositide-3-kinase regulatory subunit 4, also known as VPS15) is a 150kD adaptor protein that enhances the lipid kinase activity of PIK3 complexes and may be involved in regulating membrane trafficking late in the endocytic pathway and autophagy (1, 2) . PIK3R4 is ubiquitously expressed and contains 7 WD repeats, 3 HEAT repeats and 1 protein kinase domain (1) . PIK3R4 is crucial for VPS34 function and is necessary for autophagosome formation in multicellular animals and reveal an important role of VPS15 in cellular protection against ubiquitin-positive protein aggregates (3-5) .