Pim 1, Active | 7742

The proto-oncogene Pim1 belongs to a family of serine/threonine protein kinases that are highly conserved through evolution in multicellular organisms. Originally identified from Moloney murine leukemia virus induced T-cell lymphomas in mice, Pim1 is involved in the control of cytokine-mediated cell proliferation, differentiation and survival of lymphoid and myeloid cells as well as others. Expression of Pim1 can be stimulated by a variety of growth factors and is regulated at four different levels: transcriptional, post-transcriptional, translational and post-translational. Accumulating data support that the expression of Pim1 is mediated through activation of the JAK/STAT pathway. Some of the substrates of Pim1 include p21 Cip1, nuclear mitotic appartus protein, PTP-U2S and Socs-1. Recently, Pim1 has been shown to enhance the activities of p100, c-Myb and Cdc 25a and in part this might explain reported effects of Pim1 on mitogenesis. Pim1 interacts with c-Myb via the DNA binding domain and regulates its transcriptional activity.

7742 | Pim 1 Active DataSheet

Biomolecule/Target: N/A

Synonyms: Pim, Serine/threonine-protein kinase Pim-1

Alternates names: Pim, Serine/threonine-protein kinase Pim-1

Taglines: A proto-oncogene

NCBI Gene ID #: 9547

NCBI Gene Symbol: CXCL14

Gene Source: N/A

Accession #: O95715

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 316 nmol phosphate incorporated into the S6K substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Biological activity: The ED50 as determined by its ability to induce calcium flux of prostaglandin E2 treated THP1 human acute monocytic leukemia cells was 1.0-10.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 62.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: Reconstitute the lyophilized recombinant human CXCL14 in sterile 18M-cm H2O not less than 100 g/ml. This can further be diluted to other aqueous buffers.

Amino acid sequence: Recombinant Human CXCL14 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 9.4 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Lys-Cys-Lys-Cys.