PIM2, Active | 7730

Baytel et al. identified a deduced 334-amino acid sequence of the clone, which showed 90% identity with the mouse Pim2 protein. Like mouse Pim2, the human protein appears to be a serine threonine kinase. Northern blot analysis detected 2 PIM2 transcripts in all tissues tested, but most abundantly in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. It was also highly expressed in human leukemic and lymphoma cell lines and a colorectal adenocarcinoma cell line. The results suggested a role for PIM2 in proliferating cells as well as during meiosis (1). Yan et al investigated potential functions for the pim family of kinases in factor-dependent murine hematopoietic cells and indicate that pim-2 functions similarly to pim-1 as a pro-survival kinase and suggest that BAD is a legitimate PIM-2 substrate (2). Hammerman et al concluded that the transcriptional induction of Pim-2 initiated a novel NF-kappaB activation pathway that regulates cell survival (3).

7730 | PIM2 Active DataSheet

Biomolecule/Target: N/A

Synonyms: PIM, Serine/threonine-protein kinase Pim-2

Alternates names: PIM, Serine/threonine-protein kinase Pim-2

Taglines: A proto-oncogene

NCBI Gene ID #: 3001

NCBI Gene Symbol: GZMA

Gene Source: N/A

Accession #: P12544

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs:

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 305 nmol phosphate incorporated into the S6K substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 61.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: Reconstitute in sterile deionized water for a stock solution.

Amino acid sequence: Recombinant Human Granzyme A is expressed from HEK 293 cells. Target protein is expressed with sequence (Glu27-Val262) of Human granzyme A (Uniprot #P12544) fused with a C-terminal His tag.