PIP1;3 | Aquaporin, plasma membrane intrinistic protein 1-3 | AS09 505

PIP1;3 | Aquaporin, plasma membrane intrinistic protein 1-3 | AS09 505 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated peptide derived from N-terminal of Oryza sativa PIP1s (PIP1-1, PIP1-2, PIP1-3)

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: ELISA (ELISA), Western blot (WB)

Related Products: collection of antibodies to plasma membrane proteins AS09 504 | Anti-PIP1;3 | aquaporin, plasma membrane intrinistic protein 1-3, rabbit antibodiesAS09 506 | Anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1, rabbit antibodiesAS09 507 | Anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1, rabbit antibodiesAS09 508 | Anti-PIP2;5 | aquaporin, plasma membrane intrinistic protein 2-5, rabbit antibodies

Recommended Dilutions: 1 : 8000 (ELISA), 1 : 2000 (WB)

Molecular weight: 26-29 kDa (Oryza sativa)

Confirmed Reactivity: Oryza sativa

Predicted Reactivity: N/A

Not reactive in: Hordeum vulgare

Additional Information: Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.

Background: PIP1s - is a plasma membrane aquaporine which facilitates trasport of water across cell membrane.

Reconstitution: For reconstitution add 100 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Plasma membrane

Research Area: Cell Biology