PIP2;2 | Plasma membrane aquaporin 2b | AS09 490

PIP2;2 | Plasma membrane aquaporin 2b | AS09 490 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from N-terminal part of Raphanus sativus PAQ2b UniProt: Q9MB99

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: ELISA (ELISA), Western blot (WB)

Related Products: collection of antibodies to plasma membrane proteins AS09 488 | Anti-PIP2;1 | aquaporin PIP2;1, rabbit antibodiesAS09 489 | Anti-PIP1;1, PIP1;2, PIP1;3 | aquaporins, rabbit antibodiesAS09 491 | Anti-PIP2;1, PIP2;2, PIP2;3 |plasma membrane intrinistic protein 2-1, 2-2, 2-3, rabbit antibodiesPlant protein extraction bufferSecondary antibodies

Recommended Dilutions: 1 : 8000 (ELISA), 1 : 2000 (WB)

Molecular weight: 30 | 28 kDa (Raphanus sativus)

Confirmed Reactivity: Arabidopsis thaliana, Fragaria ananassa, Raphanus sativus

Predicted Reactivity: Brassica sp. Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Antiboy will weakly react with PIP2;1 and theoretically with PIP2;3, however this protein is very scarcely expressed in plant tissues. The mRNA content is 1 % of that for PIP2;2, therefore reactivity to PIP2;3 was neglegible in our experiments.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.

Background: PIP2;2 is a plasma membrane aquaporin.

Reconstitution: For reconstitution add 100 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT2G37170

Category: Plasma membrane

Research Area: Cell Biology