PIP2;5 | aquaporin, plasma membrane intrinistic protein 2-5 | AS09 508

PIP2;5 | aquaporin, plasma membrane intrinistic protein 2-5 | AS09 508 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated peptide derived from Oryza sativa PIP2-5 UniProt: Q8GRI8

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: ELISA (ELISA), Western blot (WB)

Related Products: collection of antibodies to plasma membrane proteins AS09 504 | Anti-PIP1;3 | aquaporin, plasma membrane intrinistic protein 1-3, rabbit antibodiesAS09 505 | Anti-PIP1s | aquaporin, rabbit antibodiesAS09 506 | Anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1, rabbit antibodiesAS09 507 | Anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1, rabbit antibodiesPlant protein extraction buffer

Recommended Dilutions: 1 : 8000 (ELISA), 1 : 2000 (WB)

Molecular weight: 29, 7 | 26-29 kDa

Confirmed Reactivity: Oryza sativa

Predicted Reactivity: Sorghum bicolor, Zea mays Species of your interest not listed? Contact us

Not reactive in: Arabidopsis thaliana, Hordeum vulgare

Additional Information: Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.

Background: PIP2;5 protein is an aquaporins which facilitates the transport of water and small neutral molecules across cell membrane.

Reconstitution: For reconstitution add 100 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Plasma membrane

Research Area: Cell Biology