PJA1 Antibody | 8069

PJA1 Antibody | 8069 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: PJA1 antibody was raised against a 19 amino acid peptide near the amino terminus of human PJA1.
The immunogen is located within amino acids 50 - 100 of PJA1.

Research Area: Cancer, Homeostasis

Tested Application: E, WB, IHC-P, IF

Application: PJA1 antibody can be used for detection of PJA1 by Western blot at 0.25 – 0.5 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: PJA1 antibody is human, mouse and rat reactive. At least two isoforms of PJA1 are known to exist; this antibody will detect both isoforms.

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 71 kDa
Observed: 71 kDa

Validation: N/A

Isoform: N/A

Purification: PJA1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PJA1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: PJA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Praja ring finger 1, E3 ubiquitin-protein ligase Praja-1, PRAJA1, RING finger protein 70, RNF70

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Ubiquitinization is an important cellular degradation process requiring sequential reactions that are mediated by three enzymes: E1, E2 and E3. PJA1, also known as Praja1 and RING finger protein 70, is a 643 amino acid E2-dependent E3-ubiquitin ligase that is abundantly expressed in the brain (1, 2) . Through interaction and activation with the E2-ubiquitin ligase UBC4, PJA1 mediates substrate-specific ubiquitination via its RING finger domain and facilitates ubiquitination (3) . Overexpression of PJA1 in gastrointestinal cancers suggests that it may be responsible for the degradation of some anti-oncogenic proteins (4, 5) .