PKAca, Active | 7743

Most of the effects of cAMP are mediated through the phosphorylation of target proteins on serine or threonine residues by the cAMP-dependent protein kinase (AMPK). The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. The mammalian catalytic subunit has been shown to consist of three PKA gene products: C-, C-, and C-. Two PKA isoforms exist, designated types I and II, which differ in their dimeric regulatory subunits, designated RI and RII, respectively. Furthermore, there are at least four different regulatory subunits: RI-, RI-, RII-, and RII-. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. The catalytic subunit C- of PKA (PKAca) is a member of the Ser/Thr protein kinase family and is a catalytic subunit C- of AMPK. Tasken et al. assigned the PKAca gene to 19p13.1 (1). Yasuda et al found that protein kinase A is required for long-term potentiation in neonatal tissue and suggested that developmental changes in synapse morphology may underlie the changes in the kinase activity (2). Skalhegg et al generated a null mutation in the major catalytic subunit of PKAca, and observed early postnatal lethality in the majority of C- knockout mice. Surprisingly, a small percentage of C- knockout mice, although runted, survived to adulthood. In these animals, compensatory increases in C- levels occurred in brain whereas many tissues, including skeletal muscle, heart, and sperm, contained less than 10% of the normal PKA activity (3).

7743 | PKAca Active DataSheet

Biomolecule/Target: N/A

Synonyms: PKA, cAMP-dependent protein kinase catalytic subunit alpha

Alternates names: PKA, cAMP-dependent protein kinase catalytic subunit alpha

Taglines: Involved in regulation of glycogen, sugar, and lipid metabolism

NCBI Gene ID #: 4803

NCBI Gene Symbol: NGF

Gene Source: N/A

Accession #: P01138

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 137 nmol phosphate incorporated into Histone H1 per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Biological activity: The ED as determined using PC12 cell line is 1.2-2 ng/ml and using DRG cell line is 0.8-1.5 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 69.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A