PKAcb, Active | 7744

Most of the effects of cAMP are mediated through the phosphorylation of target proteins on serine or threonine residues by the cAMP-dependent protein kinase (PKA). The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. The mammalian catalytic subunit has been shown to consist of three PKA gene products: C-, C-, and C-. Two PKA isoforms exist, designated types I and II, which differ in their dimeric regulatory subunits, designated RI and RII, respectively. Furthermore, there are at least four different regulatory subunits: RI-, RI-, RII-, and RII-. The cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. The catalytic subunit C- of PKA (PKAcb) is a member of the Ser/Thr protein kinase family and is a catalytic subunit C- of AMPK. Berube et al. assigned the PKAcb to human chromosome 1 by Southern blot analysis of somatic cell hybrids (1) and Simard et al located it to 1p36.1 by in situ hybridization (2).

7744 | PKAcb Active DataSheet

Biomolecule/Target: N/A

Synonyms: PKA, cAMP-dependent protein kinase catalytic subunit alpha

Alternates names: PKA, cAMP-dependent protein kinase catalytic subunit alpha

Taglines: Involved in regulation of glycogen, sugar, and lipid metabolism

NCBI Gene ID #: 4803

NCBI Gene Symbol: NGF

Gene Source: N/A

Accession #: P01138

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 342 µmol phosphate incorporated into CREBtide substrate per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Biological activity: The ED as determined by the ability to stimulate neurite growth in chick E9 DRG cells is <1.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 65.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: Reconstituted human NGF- should be stored in working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HAS or BSA). Avoid freeze/thaw cycles.

Amino acid sequence: N/A