PKC iota, Active | 7705

PKC is a member of the protein kinase C family of serine-threonine kinases. The amino acid sequence of PKC showed greatest homology to PKC zeta, with 72% identity overall rising to 84% in the catalytic domain. In contrast, the homology of PKC to the other isoforms is less pronounced, with < 53% identity even in the highly conserved catalytic region. PKC transcript is present predominantly in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. PKC is stimulated by tumor necrosis factor (TNF-) and is required for the activation of NF-kB by this cytokine. Cell transfections with a PKC dominant negative mutant abolished TNF--induced NFkB-dependent transcription. PKC can modify vulnerability of neural cells to apoptosis induced by amyloid -peptide (ABP), a cytotoxic peptide linked to neuronal degeneration in Alzheimer's disease. Associated with the increased resistance to apoptosis are improved mitochondrial function and reduced activity of caspases. In addition, ABP-induced increases in levels of oxidative stress and intracellular calcium levels were attenuated in cells overexpressing PKC .

7705 | PKC iota Active DataSheet

Biomolecule/Target: N/A

Synonyms: PKC, Protein kinase C iota

Alternates names: PKC, Protein kinase C iota

Taglines: Phosphorylate a wide variety of protein targets

NCBI Gene ID #: 4803

NCBI Gene Symbol: NGF

Gene Source: N/A

Accession #: P01138

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 664 nmol phosphate incorporated into CREBtide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay in 25µl reaction volume).

Biological activity: The ED as determined by the ability to stimulate neurite growth in chick E9 DRG cells is <1.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: ~98.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: Reconstituted human NGF- should be stored in working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HAS or BSA). Avoid freeze/thaw cycles.

Amino acid sequence: N/A