Additional Information
Size:  | 
                                                    100 µl  | 
                                                
Target:  | 
                                                    PKC theta (pSer695)  | 
                                                
Conjugate:  | 
                                                    APC  | 
                                                
Research Area:  | 
                                                    Cell Signaling | Protein Phosphorylation | Serine / Threonine Kinases | PKC | Cell Signaling | Metabolism | Metabolism processes | Hypoxia | Cancer | Cancer Metabolism | Response to Hypoxia  | 
                                                
Alternative Name:  | 
                                                    PKC θ Antibody, PRKCQ Antibody, PRKCT Antibody, nPKC-theta Antibody, Protein kinase C theta type Antibody, EC:2.7.11.13 Antibody, , KPCT_HUMAN antibody, MGC126514 antibody, MGC141919 antibody, nPKC theta antibody, nPKC-theta antibody, nPKCtheta antib  | 
                                                
Category:  | 
                                                    Antibodies  | 
                                                
Product Type:  | 
                                                    Polyclonal Antibody  | 
                                                
Immunogen:  | 
                                                    Synthetic phospho-peptide from human PKC theta around the phosphorylation site of serine 695 (pSer695).  | 
                                                
Immunogen Species:  | 
                                                    Human  | 
                                                
Applications:  | 
                                                    WB, IHC, ELISA  | 
                                                
Host:  | 
                                                    Rabbit  | 
                                                
Isotype:  | 
                                                    IgG  | 
                                                
Species Reactivity:  | 
                                                    Human, Mouse  | 
                                                
Antibody Dilution:  | 
                                                    WB (1:250-1:500); IHC (1:25-1:50); ELISA (1:2500); optimal dilutions for assays should be determined by the user.  | 
                                                
Purification:  | 
                                                    Peptide Affinity Purified  | 
                                                
Storage Buffer:  | 
                                                    PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide  | 
                                                
Concentration:  | 
                                                    N/A  | 
                                                
Specificity:  | 
                                                    Detects endogenous levels of PKC theta only when phosphorylated at serine 695 (pSer695).  | 
                                                
Storage:  | 
                                                    -20ºC  | 
                                                
Shipping:  | 
                                                    Blue Ice or 4ºC  | 
                                                
Certificate of Analysis:  | 
                                                    A 1:500 dilution of SPC-1419 was sufficient for detection of PKC theta (pSer695) in 10 µg of human jurkat cell lysates by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody.  | 
                                                
Cellular Localization:  | 
                                                    Plasma Membrane, Cell Membrane, Peripheral Membrane Protein, Cytoplasm, Cytoskeleton, Microtubule Organizing Center, Cytosol, Immunological Synapse, Aggresome, Intracellular  | 
                                                
Tissue Specificity:  | 
                                                    Expressed in skeletal muscle, T-cells, megakaryoblastic cells and platelets.  | 
                                                
Accession Number:  | 
                                                    NP_001229342.1  | 
                                                
Gene ID:  | 
                                                    5588  | 
                                                
Swiss-Prot:  | 
                                                    Q04759  | 
                                                
Field of Use:  | 
                                                    For in vitro research use only.  |