PKCa, Active | 7714

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. PKC- has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Coussens defined a new family of PKC-related genes and termed , , and (1). Latos-Bielenska refined the assignment of PRKCA1 to 17q22-q23.2 (2). Braz identified PKC- as a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes in study of knockout mice (3).

7714 | PKCa Active DataSheet

Biomolecule/Target: N/A

Synonyms: PKC, Protein kinase C alpha (PKC),

Alternates names: PKC, Protein kinase C alpha (PKC),

Taglines: Phosphorylate a wide variety of protein targets

NCBI Gene ID #: 18049

NCBI Gene Symbol: NGF

Gene Source: N/A

Accession #: P01139

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 69 nmol phosphate incorporated into CREBtide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Biological activity: This NGF- is fully biologically active when compared to standards. The ED as measured by the dose-dependent induction of human TF-1 cell proliferation is typically 0.1–1 ng/ml

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 103.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml containing 1% HSA or BSA. The solution can be diluted into other aqueous buffers and stored at 4°C for 2-7 days or store at –20°C for future use.

Amino acid sequence: N/A