PKCzeta, Active | 7718

PKC (PKC zeta) is an atypical isoform of the PKC family. PKC is found in both particulate and soluble fractions and cannot be activated by phorbol ester. Treatment of cells with phorbol ester which activates PKC, , , and isoforms in NIH3T3 cells significantly reduced proliferation of cells. Overexpression of PKC and subsequent phorbol ester treatment abolished phorbol ester-induced reduction in cell proliferation (1). Overexpression of PKC also potentiated phorbol ester-induced mitogen-activated protein (MAP) kinase activation in a PKC-dependent manner. The effects of PKC overexpression on proliferation and MAP kinase activation are proportional to the levels of PKC expression. PKC as an upstream modulator of p70S6K, an important regulator of cell proliferation (2). Kinase-inactive PKC mutant antagonized activation of p70S6K by epidermal growth factor, PDK-1, and activated Cdc42 and PI3-K. Overexpression of a constitutively active PKC mutant (myristoylated PKC [myr-PKCzeta]) only modestly activated p70S6K but this mutant cooperated with PDK-1 for the activation of p70S6K. PDK-1-induced activation of a C-terminal truncation mutant of p70S6K was also enhanced by myr-PKC. p70S6K can associate with both PDK-1 and PKCz in vivo in a growth factor-independent manner, while PDK-1 and PKC can also associate with each other, suggesting the existence of a multimeric PI3-K signalling complex.

7718 | PKCzeta Active DataSheet

Biomolecule/Target: N/A

Synonyms: PKC, PKC, Protein kinase C zeta

Alternates names: PKC, PKC, Protein kinase C zeta

Taglines: Phosphorylate a wide variety of protein targets

NCBI Gene ID #: 18205

NCBI Gene Symbol: NTF3

Gene Source: N/A

Accession #: P20181

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 93 nmol phosphate incorporated into CREBtide per minute per mg protein at 300C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Biological activity: The ED as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of 20-50 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 93.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 30% glycerol).

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml.

Amino acid sequence: N/A