PKD2, Active | 7711

PKD2 is a novel phorbol ester- and growth factor-stimulated serine/threonine kinase that contains two cysteine-rich motifs at the N terminus, a pleckstrin homology domain, and a catalytic domain (1). It exhibits the strongest homology to the serine/threonine protein kinases PKD/PKCmu and PKCnu, particularly in the duplex zinc finger-like cysteine-rich motif, in the pleckstrin homology domain and in the protein kinase domain. The PKD family of enzymes have been implicated in very diverse cellular functions, including Golgi organization and plasma membrane directed transport, metastasis, immune responses, apoptosis and cell proliferation (2).PKD2 can be activated by phorbol esters both in vivo and in vitro but also by gastrin via the cholecystokinin/CCK(B) receptor in human gastric cancer cells stably transfected with the CCK(B)/gastrin receptor (AGS-B cells) (3). Gastrin-stimulated PKD2 activation involves a heterotrimeric G (q) protein as well as the activation of phospholipase C. Furthermore, PKD2 can be activated by classical and novel members of the protein kinase C (PKC) family such as PKC , PKC epsilon, and PKC eta implicating PKD2 to be a downstream target of specific PKCs upon the stimulation of AGS-B cells with gastrin . PKD2 is predominantly cytoplasmic and stimulation of cells with the G protein-coupled receptor agonist neurotensin induces a rapid and reversible plasma membrane translocation of PKD2 by a mechanism that requires PKC activity (4). In contrast to the other PKD isoenzymes, PKD2 activation does not induce its redistribution from the cytoplasm to the nucleus.

7711 | PKD2 Active DataSheet

Biomolecule/Target: N/A

Synonyms: PKD, [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2

Alternates names: PKD, [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2

Taglines: Inactivates the enzyme pyruvate dehydrogenase

NCBI Gene ID #: 18205

NCBI Gene Symbol: NTF3

Gene Source: N/A

Accession #: P20181

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 259 nmol phosphate incorporated into CREBtide substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Biological activity: The ED as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of 20-50 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: ~130.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml.

Amino acid sequence: N/A