118
PKM2 protein (His tag) | 80R-1223
- SKU:
- 118-80R-1223-GEN
- Availability:
- Usually ships in 5 working days
Description
PKM2 protein (His tag) | 80R-1223 |
Activity Code: ACTIVE
Product Type: Proteins
Product Subtype: Conjugated Proteins
Research Area: Protein Modification & Stress Response
Short Description: Purified recombinant Human PKM2 protein
Immunogen: N/A
Host: N/A
Specificity: N/A
Cross Reactivity: N/A
Isotype: N/A
IClone: N/A
Species: Human
Residues: 1-531 amino acids: MGSSHHHHHH SSGLVPRGSH MSKPHSEAGT AFIQTQQLHA AMADTFLEHM CRLDIDSPPI TARNTGIICT IGPASRSVET LKEMIKSGMN VARLNFSHGT HEYHAETIKN VRTATESFAS DPILYRPVAV ALDTKGPEIR TGLIKGSGTA EVELKKGATL KITLDNAYME KCDENILWLD YKNICKVVEV GSKIYVDDGL ISLQVKQKGA DFLVTEVENG GSLGSKKGVN LPGAAVDLPA VSEKDIQDLK FGVEQDVDMV FASFIRKASD VHEVRKVLGE KGKNIKIISK IENHEGVRRF DEILEASDGI MVARGDLGIE IPAEKVFLAQ KMMIGRCNRA GKPVICATQM LESMIKKPRP TRAEGSDVAN AVLDGADCIM LSGETAKGDY PLEAVRMQHL IAREAEAAIY HLQLFEELRR LAPITSDPTE ATAVGAVEAS FKCCSGAIIV LTKSGRSAHQ VARYRPRAPI IAVTRNPQTA RQAHLYRGIF PVLCKDPVQE AWAEDVDLRV NFAMNVGKAR GFFKKGDVVI VLTGWRPGSG FTNTMRVVPV P
Tag/conjugate: His tag
Protein Type: Recombinant
Expression System: E.coli
Grade & Purity: > 95% pure
Methode of Purification: N/A
Source: N/A
Concentration: 1 mg/ml
Form & Buffer: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol.
Storage: Store at 4 deg C for short term storage (1/2 weeks) . Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Shipping Information: *Blue Ice*
Applications: SDS-PAGE
Bioactivity: N/A
Usage Recommendations: N/A
Assay Information: 1. Prepare a 150ul reaction mixture into a suitable container. Reaction mixture: 72mM Imidazole ( pH 7.5), 7.6mM ADP, 15mM MgCl2, 72mM KCl, 0.2mM Beta-NADH. 2. Incubate for 10 min at 37C. 3. Add 50ul of 20 units/ml LDH, 50ul of 5mM PEP, and incubate for 10 min at 37C. 4. Add 50ul of recombinant PKM2 protein (in 0.1% BSA / PBS) with various concentrations (0.03ug -1ug) . 5. Read the decrease in A340nm in kinetic mode for 10 minutes.