PKN1/2 (Phospho-T774/T816) polyclonal Antibody | BS64582

PKN1/2 (Phospho-T774/T816) polyclonal Antibody | BS64582 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB

Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200

Background: Protein kinase N (PKN) is a fatty acid-activated serine/threonine kinase whose catalytic domain exhibits homology with that of the PKC family. PKN associates with Rho via its amino terminus, is activated in a GTP-dependent manner and phosphorylates the head-rod domain of neurofilament protein. A second protein, rhophilin, exhibits 40% sequence identity with the amino terminal Rho binding domain. The enzymatic activity of rhophilin has not been demonstrated and it is possible that it acts through the recruitment of cytoskeletal components that initiate a kinase signaling cascade. Citron interacts specifically with active Rho and Rac1 but not Cdc42. Citron exhibits a distinctive protein organization and little homology with the Rho binding domains of PKN and rhophilin.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PKN1/2 (Phospho-T774/T816)  polyclonal Antibody detects endogenous levels of PKN1/2 protein only when phosphorylated at Thr774/Thr816.

Molecular Weight: ~ 130 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Serine/threonine-protein kinase N1; Protease-activated kinase 1; PAK-1; Protein kinase C-like 1; Protein kinase C-like PKN; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; Serine-threonine protein kinase N; PAK1; PKN; PRK1; PRKCL1; Serine/threonine-protein kinase N2; PKN gamma; Protein kinase C-like 2; Protein-kinase C-related kinase 2; PKN2; PRK2; PRKCL2

Immunogen: Synthetic phosphopeptide derived from human PKN1/2 around the phosphorylation site of Threonine 774/Threonine 816.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: