PLIN1-Myc Stable Expressing 3T3-L1 Cell Line | T6289

PLIN1-Myc Stable Expressing 3T3-L1 Cell Line | T6289

3T3-L1 cells were stably transfected with the pLXSN-Flag-Plin1 WT and pBABEpuro-myc-Plin1 WT vectors to establish the PLIN1-Myc Stable Expressing 3T3-L1 Cell Line. The cells are G418 and puromycin-resistant. Lipid droplet-associated protein, Perilipin 1, or PLIN1, has a role in lipid metabolism and adipocyte lipolysis. This cell line may be used to study processes in lipolysis and lipid metabolism pathway.

Biosafety:

II

Organism:

Mouse (M. musculus)

Source Organ:

Embryo

Growth Properties:

Adherent

Morphology:

Fibroblast

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 - 48 hours

Seeding Density:

N/A

Markers:

Perilipin, G418-resistance, Puromycin-resistance, and Myc

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

Note: selection antibiotics should not be added upon thawing or for routine cell propagation. Let cells recover first after thawing, before adding the selection antibiotics.
Cells exhibit contact inhibition.
The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Western blot

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C