POLI polyclonal Antibody | BS61707

POLI polyclonal Antibody | BS61707 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Application: WB IHC

Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200

Background: DNA polymerase activity is essential for replication, repair, recombination and mutagenesis. DNA polymerases can often bypass DNA lesions that block DNA replication, thereby allowing the replication of damaged DNA. One such DNA polymerase is the distributive enzyme DNA pol ι (pol iota), which is encoded by the POLI gene. POLI is located on human chromosome 18q21.2, a region often implicated in the etiology of many human cancers. At thymine templates, DNA Pol ι is highly error-prone when replicating undamaged DNA in that it favors the misincorporation of guanine over the correct nucleotide, adenosine. DNA Pol ι also promotes the replication of damaged DNA by misincorporating deoxynucleotides opposite DNA lesions. DNA Pol ι acts sequentially with DNA Pol ζ, which is essential for damageinduced mutagenesis, to complete the DNA lesion bypass. Therefore, replication involving DNA Pol ι is likely to be highly mutagenic.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: POLI polyclonal Antibody detects endogenous levels of POLI protein.

Molecular Weight: ~ 83 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: DNA polymerase iota; Eta2; RAD30 homolog B; POLI; RAD30B

Immunogen: Synthetic peptide, corresponding to amino acids 630-680 of Human POLI.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: