POLN Antibody (C-term) [APR31285G]

POLN Antibody (C-term) [APR31285G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: M

Specificity: This POLN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 651-679 amino acids from the C-terminal region of human POLN.

Cellular Localisation: Nucleus.

Molecular Weight: 100307

Clone: Polyclonal

Gene Name: POLN

Gene ID: 353497

Function: DNA polymerase with very low fidelity that catalyzes considerable misincorporation by inserting dTTP opposite a G template, and dGTP opposite a T template (PubMed:16787914, PubMed:17118716) . Is the least accurate of the DNA polymerase A family (i.e. POLG, POLN and POLQ) (PubMed:17118716) . Can perform accurate translesion DNA synthesis (TLS) past a 5S-thymine glycol. Can perform efficient strand displacement past a nick or a gap and gives rise to an amount of product similar to that on non-damaged template. Has no exonuclease activity (PubMed:16787914) . Error-prone DNA polymerase that preferentially misincorporates dT regardless of template sequence (PubMed:25775266) . May play a role in TLS during interstrand cross-link (ICL) repair (PubMed:19908865) . May be involved in TLS when genomic replication is blocked by extremely large major groove DNA lesions. May function in the bypass of some DNA-protein and DNA-DNA cross-links. May have a role in cellular tolerance to DNA cross-linking agents (PubMed:20102227) . Involved in the repair of DNA cross-links and double-strand break (DSB) resistance. Participates in FANCD2-mediated repair. Forms a complex with HELQ helicase that participates in homologous recombination (HR) repair and is essential for cellular protection against DNA cross-links (PubMed:19995904) .

Summary: Tissue Location: Highly expressed in testis and heart. Weakly expressed in skeletal muscle.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:500

Synonyms: DNA polymerase nu, POLN