POLR2A Antibody | 13-946

POLR2A Antibody | 13-946 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat, Pig

Homology: N/A

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2A (NP_000928.1) .

Research Area: Neuroscience

Tested Application: WB, IHC, IF, IP, ChIP

Application: WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
ChIP: 1:20 - 1:50

Specificiy: N/A

Positive Control 1: HeLa

Positive Control 2: BT-474

Positive Control 3: H460

Positive Control 4: Jurkat

Positive Control 5: Mouse testis

Positive Control 6: Mouse brain

Molecular Weight: Observed: 250kDa

Validation: N/A

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration: N/A

Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.

Alternate Name: POLR2A, HRPB220, HsRPB1, POLR2, RpIILS, RPOL2, RNA Polymerase II, RPO2, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.