Poly (ADP-ribose) Antibody [10H] | 36-169

Poly (ADP-ribose) Antibody [10H] | 36-169 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Drosophila, Human, Mouse, Rat

Homology: N/A

Immunogen: Purified poly (ADP-ribose) .

Research Area: Apoptosis, Cancer, Innate Immunity, Obesity

Tested Application: Flow, ICC, IHC, WB

Application: Flow Cytometry: (see A. Kunzmann, et al.; Immun. Ageing 3, 8 (2006) ) . Immunocytochemistry: (5-20ug/ml) . Immunohistochemistry (paraffin sections) : Dilute in 5% non fat dried milk in PBS to a final concentration of 5-20ug/ml) . Western Blot: Incubate 2.5ug/ml in PBS, 0.05% Tween20, 5% non fat dried milk. Optimal conditions must be determined individually for each application.

Specificiy: Recognizes poly (ADP-ribose) synthesized by a broad range of PARPs (poly (ADP-ribose) polymerases) , including human, mouse, rat or Drosophila PARP enzymes.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: >95% (SDS-PAGE)

Clonality: Monoclonal

Clone: 10H

Isotype: IgG3, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Liquid. Containing 50mM HEPES, pH 7.4, 100mM NaCl, 1% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: Stable for at least 1 year after receipt when stored at -80˚C.

Alternate Name: N/A

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Processes such as transcription, repair and replication that require efficient DNA recognition are dependent on modulation of chromatin structure. Chromatin relaxation is a critical event that occurs during DNA repair and is associated with the negatively charged polymer of adenosine 5'-diphosphate (ADP) -ribose (PAR) . PAR is synthesized from nicotinamide adenine dinucleotide (NAD+) by the poly (ADP-ribose) polymerase protein family (PARPs) , of which PARP-1 (and to a lesser extent PARP-2) respond to DNA-strand breaks. PARP-1 is selectively activated by DNA strand breaks to catalyze the addition of long branched chains of PAR to a variety of nuclear proteins, most notably PARP itself. The amount of PAR formed in living cells with DNA damage is commensurate with the extent of the damage. Under DNA damage conditions, PAR undergoes a rapid turnover, with a half-life in the range of minutes, as PAR is rapidly hydrolyzed and converted to free ADP-ribose by the enzyme poly (ADP-ribose) glycohydrolase (PARG) . PAR regulates not only cell survival and cell-death programmes, but also an increasing number of other biological functions with which novel members of the PARP family have been associated. These include transcriptional regulation, cell division, intracellular trafficking, inflammation and energy metabolism.