Poly(I:C) | B2989

Poly(I:C) is a double-stranded homopolymer of polyinosinic acid and polycytidylic acid. Transfection of Poly(I:C) (1 μg/ml) into NIT 1 cells has been used as a model of intracellular double-stranded RNA-induced beta-cell apoptosis. Poly(I:C) induced apoptosis in 45% of the cells, 18h after transfection. Maturation mixtures containing various cytokines, IFN-γ and TLR3 agonist poly(I:C) in combination with TLR7/8 agonists R848 or CL075 yield 3-d mature dendritic cells that secrete high levels of IL-12(p70) and show strong chemotaxis to CCR7 ligands. Epigenetic modulation of cardiac progenitor cells with Poly(I:C) accelerates maturation of cardiomyocytes.

Poly(I:C) is a double-stranded homopolymer of polyinosinic acid and polycytidylic acid. Transfection of Poly(I:C) (1 μg/ml) into NIT 1 cells has been used as a model of intracellular double-stranded RNA-induced beta-cell apoptosis. Poly(I:C) induced apoptosis in 45% of the cells, 18h after transfection. Maturation mixtures containing various cytokines, IFN-γ and TLR3 agonist poly(I:C) in combination with TLR7/8 agonists R848 or CL075 yield 3-d mature dendritic cells that secrete high levels of IL-12(p70) and show strong chemotaxis to CCR7 ligands. Epigenetic modulation of cardiac progenitor cells with Poly(I:C) accelerates maturation of cardiomyocytes.

B2989 | Poly(I:C) DataSheet

Alternate Name/Synonyms: [(2R,3S,4R,5R)-5-(4-amino-2-oxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl dihydrogen phosphate--[(2R,3S,4R,5R)-3,4-dihydroxy-5-(6-oxo-1H-purin-9-yl)oxolan-2-yl]methyl dihydrogen phosphate; Polyinosinic acid-polycytidylic acid; Poly I:poly C; Polyinosinic acid, homopolymer, complex with polycytidylic acid, homopolymer (1:1)

Appearance: White to off-white powder

Formulation:

CAS Number: 24939-03-5

Structure Available?: True

Peptide sequence:

Salt Form: False

Molecular Formula: (C₁₀H₁₃N₄O₈P)x·(C₉H₁₄N₃O₈P)x

Molecular Weight: 671.4

Cell-Permeable?: True

Purity: >95%

Solubilities: To prevent denaturation, this product should be reconstituted in solutions with physiological salt concentrations. Ultrasonication or gentle warming can be carried out for improving solubility. ~3 mg/ml in Water

Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.

Country of Origin: USA

Tag Line: A double-stranded homopolymer and Toll-like receptor 3 (TLR3) agonist

MDL Number: MFCD00131957

PubChem CID: 135618150

SMILES: C1=CN(C(=O)N=C1N)C2C(C(C(O2)COP(=O)(O)O)O)O.C1=NC2=C(C(=O)N1)N=CN2C3C(C(C(O3)COP(=O)(O)O)O)O

InChi: InChI=1S/C10H13N4O8P.C9H14N3O8P/c15-6-4(1-21-23(18,19)20)22-10(7(6)16)14-3-13-5-8(14)11-2-12-9(5)17;10-5-1-2-12(9(15)11-5)8-7(14)6(13)4(20-8)3-19-21(16,17)18/h2-4,6-7,10,15-16H,1H2,(H,11,12,17)(H2,18,19,20);1-2,4,6-8,13-14H,3H2,(H2,10,11,15)(H2,16,17,18)/t4-,6-,7-,10-;4-,6-,7-,8-/m11/s1

InChi Key: ACEVNMQDUCOKHT-ZLOOHWKQSA-N