POU2F3 Antibody | 7795

POU2F3 Antibody | 7795 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: POU2F3 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human POU2F3.
The immunogen is located within amino acids 370 - 420 of POU2F3.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: POU2F3 antibody can be used for detection of POU2F3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Specificiy: POU2F3 antibody is human, mouse and rat reactive. At least two isoforms are known to exist. This antibody will recognize both isoforms. POU2F3 antibody is predicted to not cross-react with other members of the POU domain class 2 family.

Positive Control 1: Cat. No. 1220 - SK-N-SH Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 48 kDa
Observed: 55 kDa

Validation: N/A

Isoform: N/A

Purification: POU2F3 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: POU2F3 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: POU2F3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: POU2F3 Antibody: PLA1, OCT11, PLA-1, Epoc-1, OCT-11, OTF-11, Skn-1a, OTF11, PLA1, POU domain, class 2, transcription factor 3, Octamer-binding protein 11, 41923

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: POU2F3, also known as Epoc-1, is a member of a family of POU domain family of transcription factors (1) . POU2F3 is expressed primarily in the epidermis and plays a critical role in keratinocyte proliferation and differentiation (1, 2) . It is a crucial transcription factor that is required for the development of sweet, umami, and bitter, but not sour taste receptor cells (3) . POU2F3 is also a candidate tumor suppressor protein, and aberrant promoter methylation of this gene may play a role in cervical cancer (4) .