PPARGC1A Antibody | 7793

PPARGC1A Antibody | 7793 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%) , Pig: (94%)

Immunogen: PPARGC1A antibody was raised against a 17 amino acid peptide near the amino terminus of human PPARGC1A.
The immunogen is located within amino acids 200 - 250 of PPARGC1A.

Research Area: Obesity

Tested Application: E, WB, IHC-P, IF

Application: WB: 2 μg/mL; IHC: 1-2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: PPARGC1A antibody is human, mouse and rat reactive. At least three isoforms of PPARGC1A are known to exist; this antibody will detect all three isoforms.

Positive Control 1: Cat. No. 1419 - Mouse Skin Tissue Lysate

Positive Control 2: Cat. No. 1302 - Human Lung Tissue Lysate

Positive Control 3: Cat. No. 1224 - Daudi Cell Lysate

Positive Control 4: Cat. No. 1204 - K562 Cell Lysate

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 91kD
Observed: 100 kD

Validation: N/A

Isoform: N/A

Purification: PPARGC1A antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PPARGC1A antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: PPARGC1A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: PPARGC1A Antibody: LEM6, PGC1, PGC1A, PGC-1v, PPARGC1, PGC-1 (alpha) , LEM6, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, Ligand effect modulator 6, PGC-1-alpha

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) , also known as LEM-6, is a transcriptional coactivator that regulates the genes involved in energy metabolism (1) . PPARGC1A interacts with PPARgamma, which permits the interaction of PPARGC1A with multiple transcription factors. PPARGC1A can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs) . It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination (2) . PPARGC1A may be also involved in the development of obesity (3) .