PPP1SS, human recombinant | P1182

PPP1CC, also known as serine/threonine-protein phosphatase PP1-gamma catalytic subunit, is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. This protein is involved in regulation of ionic conductances and long-term synaptic plasticity and may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Recombinant human PPP1CC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

P1182 | PPP1SS human recombinant DataSheet

Biomolecule/Target: PPP1SS

Synonyms: Serine/threonine-protein phosphatase PP1-gamma catalytic subunit, PP1gamma, PPP1G

Alternates names: Serine/threonine-protein phosphatase PP1-gamma catalytic subunit, PP1gamma, PPP1G

Taglines: Serine/threonine-protein phosphatase PP1-gamma catalytic subunit

NCBI Gene ID #: 8602

NCBI Gene Symbol: OPG

Gene Source: Human

Accession #: O14790

Recombinant: Yes

Source: E.coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its ability to hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37°C.

Biological activity: Determined by its ability to neutralize the stimulation of U937 cells treated with 10 ng/ml of sRANKL.

Results: > 700 units/mg

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 39.1 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In 20mM Tris-HCl buffer (pH8.0) containing 50% glycerol, 0.2M NaCl, 2mM DTT

Reconstitution Instructions: Reconstitute in 5 mM Tris, pH 7.5 to a concentration of 0.1-1.0 mg/ml. The solution can be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A