PPP3CA Antibody (C-term) [APR14378G]

PPP3CA Antibody (C-term) [APR14378G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This PPP3CA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-466 amino acids from the C-terminal region of human PPP3CA.

Cellular Localisation: Cytoplasm. Cell membrane; Peripheral membrane protein. Cell membrane, sarcolemma {ECO:0000250|UniProtKB:P63329}. Cytoplasm, myofibril, sarcomere, Z line {ECO:0000250|UniProtKB:P63329}. Cell projection, dendritic spine. Note=Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle (By similarity) . Recruited to the cell membrane by scaffold protein AKAP5 following L-type Ca (2+) - channel activation (PubMed:22343722) . {ECO:0000250|UniProtKB:P63329, ECO:0000269|PubMed:22343722}

Molecular Weight: 58688

Clone: Polyclonal

Gene Name: PPP3CA (HGNC:9314)

Gene ID: 5530

Function: Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca (2+) -mediated signals (PubMed:15671020, PubMed:18838687, PubMed:19154138, PubMed:23468591) . Many of the substrates contain a PxIxIT motif and/or a LxVP motif (PubMed:17498738, PubMed:17502104, PubMed:23468591, PubMed:27974827, PubMed:22343722) . In response to increased Ca (2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation (PubMed:15671020) . In response to increased Ca (2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion (PubMed:18838687) . Dephosphorylates heat shock protein HSPB1 (By similarity) . Dephosphorylates and activates transcription factor NFATC1 (PubMed:19154138) . In response to increased Ca (2+) levels, regulates NFAT-mediated transcription probably by dephosphorylating NFAT and promoting its nuclear translocation (PubMed:26248042) . Dephosphorylates and inactivates transcription factor ELK1 (PubMed:19154138) . Dephosphorylates DARPP32 (PubMed:19154138) . May dephosphorylate CRTC2 at 'Ser-171' resulting in CRTC2 dissociation from 14-3-3 proteins (PubMed:30611118) . Dephosphorylates transcription factor TFEB at 'Ser-211' following Coxsackievirus B3 infection, promoting nuclear translocation (PubMed:33691586) .

Summary: Tissue Location: Expressed in keratinocytes (at protein level) .

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, CAM-PRP catalytic subunit, Calmodulin-dependent calcineurin A subunit alpha isoform, PPP3CA, CALNA, CNA