PRC1 Antibody | 6821

PRC1 Antibody | 6821 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: PRC1 antibody was raised against a 19 amino acid synthetic peptide near the center of human PRC1.
The immunogen is located within amino acids 350 - 400 of PRC1.

Research Area: Homeostasis

Tested Application: E, WB, IHC-P, IF

Application: PRC1 antibody can be used for detection of Prc1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: At least three alternatively spliced transcript variants encoding distinct isoforms have been observed.

Positive Control 1: Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: PRC1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PRC1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: PRC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: PRC1 Antibody: ASE1, Protein regulator of cytokinesis 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: PRC1 Antibody: PRC1 (Protein Regulator of Cytokinesis 1) is a key regulator of cytokinesis and has been identified as a substrate for several cyclin-dependent kinases (CDKs) . High levels of PRC1 expression occurs during S and G2/M and drops dramatically after mitosis exit and G1 entry. It is located in the nucleus during interphase and binds to the midzone of mitotic spindles during anaphase and is localized to the cell midbody during cytokinesis. Depletion of PRC1 has been shown to prevent cellular cleavage, but it has no effect on nuclear division, demonstrating the importance of PRC1 in mitosis.