PRDM14 Antibody | 42-423

PRDM14 Antibody | 42-423 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow

Immunogen: The immunogen for this antibody is: YRDKHLKYTPCVDK

Research Area: Other

Tested Application: E, IF, Flow

Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 40kDa band in Human Heart lysates after 0.1-1ug/ml antibody staining and this band was successfully blocked by incubation with the immunizing peptide. Preliminary experiments also gave an approxand an approx 48-50kDa band in Human Lung and Kidney lysates and in nuclear lysates of cell liines Jurkat, HEK293 and MCF7. Please note that currently we cannot find an explanation in the literature for these bands we observe given the calculated size of 64.1kDa according to Human NP_078780.1.Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: PRDM14, PR domain containing 14, MGC59730, PFM11, PR domain zinc finger protein 14, PR domain-containing protein 14, PR-domain zinc finger protein 14

User Note: N/A

BACKGROUND: N/A