ProNGF, human recombinant | 4274

The precursor form of the nerve growth factor (proNGF) like its mature form is characterized by the cystine knot motif consisting of three cystine bridges, whereas proneurotrophins and mature neurotrophins elicit opposite biological effects. ProNGF functions preferentially via the complex of pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin inducing neuronal apoptosis and contributing to age- and disease-related neurodegeneration.

4274 | ProNGF human recombinant DataSheet

Biomolecule/Target: ProNGF

Synonyms: N/A

Alternates names: CT-1

Taglines: Neuronal apoptosis inducer

NCBI Gene ID #: 13019

NCBI Gene Symbol: CT1

Gene Source: Mouse

Accession #: Q60753

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is 1.0 ng/ml, corresponding to a specific activity of 1 x 106 units/mg.

Biological activity: Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is 1.0 ng/ml, corresponding to a specific activity of 1 x 106 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 21.3 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 0.5 mg/ml in 5 mM Tris, pH 8.0

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: SQREGSLEDH QTDSSISFLP HLEAKIRQTH NLARLLTKYA EQLLEEYVQQ QGEPFGLPGF SPPRLPLAGL SGPAPSHAGL PVSERLRQDA AALSVLPALL DAVRRRQAEL NPRAPRLLRS LEDAARQVRA LGAAVETVLA ALGAAARGPG PEPVTVATLF TANSTAGIFS AKVLGFHVCG LYGEWVSRTE GDLGQLVPGG VA