Propidium Iodide | 1056

Propidium Iodide (PI) binds to double-stranded DNA, but it can only cross the plasma membrane of nonviable cells. When viewed using a fluorescence microscope with a rhodamine filter, cells that have lost membrane integrity will show red PI staining throughout the nuclei. For analysis by flow cytometry, the PI staining can be monitored in FL2 channel.

Propidium iodide is a useful luorescent stain for nucleic acids.

1056 | Propidium Iodide DataSheet

Alternate Name/Synonyms: 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide, PI

Appearance: Liquid

Formulation: PBS ( 1 mg/ml)

CAS Number: 25535-16-4

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₂₇H₃₄I₂N₄

Molecular Weight: 668.39

Cell-Permeable?: No

Purity:

Solubilities: N/A

Handling: Protect from light and moisture

Country of Origin: USA

Tag Line: A nucleic acid stain

MDL Number: MFCD00011921

PubChem CID: 104981

SMILES: CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-]

InChi: InChI=1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

InChi Key: XJMOSONTPMZWPB-UHFFFAOYSA-M