Protein G-Biotin | 6512

Protein G binds to the constant region of many species of immunoglobulin G. Protein G can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. ’s Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity. The 6-His-tag on N-terminus can be used for affinity purification or protein G detection using anti-His-tag antibodies. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. The Protein G-Biotin is using purified recombinant protein G biotinylated with ~2 biotins per protein G molecule.

6512 | Protein G-Biotin DataSheet

Biomolecule/Target: N/A

Synonyms: Protein G

Alternates names: Cerebral dopamine neurotrophic factor, ARMETL1

Taglines: Genetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies

NCBI Gene ID #: 441549

NCBI Gene Symbol: CDNF

Gene Source: Human

Accession #: Q49AH0

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 15-25 µg/ml.

Biological activity: Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 15-25 µg/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 18.5 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.0.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MQEAGGRPGA DCEVCKEFLN RFYKSLIDRG VNFSLDTIEK ELISFCLDTK GKENRLCYYL GATKDAATKI LSEVTRPMSV HMPAMKICEK LKKLDSQICE LKYEKTLDLA SVDLRKMRVA ELKQILHSWG EECRACAEKT DYVNLIQELA PKYAATHPKT EL