Description
Proteinase K is a kind of high activity proteinase of subtilisin, which is used to degrade proteins in biological samples. It can be used to digest various proteins, and it can be used in a variety of molecular biology, cell biology and other related experiments, such as genomic DNA extraction, enzyme digestion and removal, cell permeability and so on.
Proteinase K is mainly used for the production of nucleic acid extraction kit, especially for nucleic acid extraction from samples of nucleic acid diagnosis and detection in vitro diagnosis (IVD) industry.
| Product | Proteinase K Lyophilized Powder |
| Cat.No. | E-IR-R109U |
| Nature | Recombination |
| Valid Period | 2~8°C for two years. RT for one year. |
| Purity | ≥95% by SDS-PAGE |
| Application | Genomic DNA Purification Kit, RNA Purification Kit, Gene Diagnostic Kit, IHC, ISH and other possible application. |
| Activity | ≥30 U/mg |
| Capacity | 15~20 kg/Month |
| Product Size | 100mg, 1g, 10g, 100g |
Product characteristics
| Recombinant expression in yeast | Activity ≥30 U/mg | DNase and RNase free | Recommend usage 50~200 μg/mL |
Activity result
Elabscience® standard production process requires that the activity ratio ≥30 U/mg. In fact, the activity ratio exceeded the expected production standard, content the application requirements of customers.
Unit definition: One unit is defined as the amount of enzyme that catalyzes the formation of 1 μmol of tyrosine per minute at pH 7.5 at 37°C.
Calibration curve
According to the absorbance A275 for the vertical axis, the different concentrations of tyrosine as the horizontal axis, the standard curve was drawn.
Detection of DNase and RNase residues
Use DNase and RNase detection kit to detect the DNase and RNase residues with different Lots of Proteinase K. The results showed that there were no DNase nor RNase in Proteinase K.
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