Proteinase K, Recombinant, Molecular Grade | 9210

A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).

9210 | Human CellExp CD163 Human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Endopeptidase K, Tritirachium alkaline proteinase

Alternates names: Placenta Growth Factor

Taglines: A serine protease which inactivates nucleases

NCBI Gene ID #: 18654

NCBI Gene Symbol: PGF

Gene Source: N/A

Accession #: P49764

Recombinant: Yes

Source: Tritirachium album

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): >30 units/mg

Biological activity: Determined by its ability to chemoattract human monocytes of using a concentration range of 0.5-10 ng/ml.

Results: 0.5-10 ng/ml

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 µmol tyrosine per min at  37°C, pH 7.5 (color by Folin-Ciocalteu reagent).

Molecular Weight: 32.6 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized with no additives

Reconstitution Instructions: Reconstitute the protein in sterile 20 mM acetic acid to a concentration of 0.1-1.0 g/l. The solution can then be diluted into other aqueous buffer

Amino acid sequence: N/A