Description
PRPF31 Antibody | 46-230 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen: The immunogen for this antibody is: KELGNSLDKCKNNEN
Research Area: Cancer
Tested Application: E, WB
Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in lysates of cell lines A431 and HeLa (calculated MW of 55.4kDa according to NP_056444.3) . Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide. Also a band of 28kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal.
Specificiy: N/A
Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: PRPF31, PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae) , DKFZp566J153, NY-BR-99, PRP31, RP11, PRP31 pre-mRNA processing factor 31 homolog (yeast) , pre-mRNA processing factor 31 homolog, pre-mRNA processing factor 31 homolog (yeast)
User Note: N/A
BACKGROUND: N/A