Description
PRPS1, also known as ribose-phosphate pyrophosphokinase 1, is ubiquitously expressed in human tissues. This enzyme catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for purine metabolism and nucleotide biosynthesis. Recombinant human PRPS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
7828 | PRPS1 human recombinant DataSheet
Biomolecule/Target: PRPS1
Synonyms: Ribose-phosphate pyrophosphokinase 1, ARTS, CMTX5, PPRibP, PRSI, DFN2, DFNX1
Alternates names: Ribose-phosphate pyrophosphokinase 1, ARTS, CMTX5, PPRibP, PRSI, DFN2, DFNX1
Taglines: An enzyme that catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate
NCBI Gene ID #: 20304
NCBI Gene Symbol: CCL5
Gene Source: Human
Accession #: P30882
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Determined by its ability to chemoattract murine T cell population using a concentration range of 1-10 ng/ml.
Results: Specific activity: > 1 Units/ml
Binding Capacity: N/A
Unit Definition: One unit will catalyze the formation of 1.0 µmole of AMP from ATP and ribose-5-phosphate per minute at pH 7.6 at 37ºC.
Molecular Weight: 36.9 kDa (338 aa, 1-318 aa + His Tag)
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1 mM DTT and 0.1 M NaCl
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.
Amino acid sequence: N/A