PsbA | D1 protein of PSII, C-terminal (chicken) | AS01 016

PsbA | D1 protein of PSII, C-terminal (chicken) | AS01 016 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020, Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others

Host: Chicken

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.

Format: Liquid

Tested Application: Western blot (WB)

Related Products: AS01 016S | PsbA protein standard for a quantitative western blotAS05 084 | Anti-PsbA C-terminal, rabbit antibodiesAS11 1786 | Anti-PsbA N-terminal, rabbit antibodiesAS10 704 | Anti/PsbA | D1 protein of PSII, DE-loop, rabbit antibodiesAS13 2669 | PsbA | D1 protein of PSII, phosphorylated, rabbit antibodyrecommended secondary antibodyPlant and algla protein extraction bufferSecondary antibodies

Recommended Dilutions: 1 :4000-1 : 8000, 5 µg of total protein, (WB)

Molecular weight: 38 | 28-30 kDa

Confirmed Reactivity: Alaria esculenta, Amphidinium carterae, Anabaena sp., Arabidopsis thaliana, Brachypodium sylvaticum, Chlamydomonas reinhardtii, Chlamydomonas raudensis (both Antarctic and mesophilic strains), Cyanophora sp., Cyanothece sp. ATCC 51142, Cynara cardunculus, Gonyaulax polyedra, Fucus vesiculosus, Horderum vulgare, Lobaria pulmonaria, Petunia sp., Pinus sylvestris, Spartina alterniflora, Synechococcus sp. PCC 7942, Triticum aestivum, Ulva sp., symbiotic dinoflagellates of Stylophora pistillata and Turbinaria reniformis, Zea mays

Predicted Reactivity: Algae (brown and red), Conifers, Cryptomonads, Legumes, Stramenopiles, Euglenoids, Prochlorophytes, Xantophytes Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions.In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.This antibody will also detect the phosphorylated form of D1as an alternate band to the main band on a high resolution gel.

Background: The PsbA (D1) protein of Photosystem II is rapidly cycled under illumination in all oxygenic photobionts. Disruption of PsbA cycling or losses of PsbA pools are central to photoinhibition of photosynthesis in cyanobacteria, algae and plants under a wide range of conditions including excess light, low temperature and UV exposure. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.

Reconstitution: N/A

Storage: Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: ATCG00020

Category: Global Antibodies

Research Area: Plant Biology, Photosynthesis