PsbA | D1 protein of PSII, C-terminal (rabbit antibody) (thylakoid membrane marker) | AS05 084

PsbA | D1 protein of PSII, C-terminal (rabbit antibody) (thylakoid membrane marker) | AS05 084 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020, Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Immunofluorescence (IF), ImmunoGold (IG), Western blot (WB)

Related Products: AS01 016S | PsbA protein standard for a quantitative western blotAS05 084PRE | PsbA | D1 protein of PSII, C-terminal, pre-immune serumAS11 1786 | Anti-PsbA N-terminal, rabbit antibodiesAS10 704 | Anti-PsbA | D1 protein of PSII, DE-loop, rabbit antibodiesAS13 2669 | Anti-PsbA | D1 protein of PSII, phosphorylated, rabbit antibodiesPlant and algal protein extraction buffer Secondary antibodies

Recommended Dilutions: 1: 500 (IF), 1: 200 (IG), 1 : 10 000 (WB)

Molecular weight: 38 | 28-30 kDa

Confirmed Reactivity: Alniaria alnifolia, Anabaena 7120, Arabidopsis thaliana, Artemisia annua, Arundo sp., Begonia sp., Cannabis sativa L., Chlamydomonas reinhardtii, Chlorella ohadii, Chromera velia, Chlorella vulgaris, Colobanthus quitensis Kunt Bartl, Coscinodiscus wailesii, Craterostigma sp., Cyanidioschyzon merolae, Cytisus cantabricus (Wilk.) Rchb. F, Desmodesmus sp., Dianthus caryophyllus, Ditylum brightwellii, Eucalyptus globulus, Fraxinus rhynchophylla, Glycine max, Halomicronema hongdechloris, Hieracium pilosella L., Hordeum vulgare, Lasallia hispanica, Lindernia sp., Manihot esculenta, Marchantia polymorpha (liverwort), Medicago truncatula, Miscanthus x giganteus, Microcystis aeruginosa, Mirkania micrantha, Nicotiana benthamiana, Nicotiana tabcum, Panicum miliaceum, Panax ginseng, Panicum maximum, Paulinella chromatophora (amoeba), Pheodactylum tricornutum CCAP 1055/1, Physcomitrium patens, Picea glauca, Pinus strobus, Pisum sativum, Prochlorococcus sp. (surface and deep water ecotype), Pisum sativum, Skeletonema costatum (diatom), Solanum lycopersicum, Spartina alterniflora, Spinacia oleracea, Spirodela polyrhiza, Symbiodinium sp, Synechococcus sp. PCC 7942, Synechococcus elongatus UTEX 2973, Synechocystis sp. 6803, Syntrichia muralis, Thalassiosira weissflogii, Tetradesmus obliquus, Triticum aestivum, Triticale, Zea may, Quercus ilex

Predicted Reactivity: Algae (brown and red), Brassica napus, Conifers, Cyanobacteria, Dictos, Cannabis sativa, Galdieria sulphuraria, Lactuca sativa, Lycopersicum esculentum, Medicago sativa, Nannochloropsis sp., Oryza sativa, Ostreococcus sp. Pisum sativum, Sesamum indicum, Thalassiosira pseudonana, Zosteria marina, Vitis vinifera cellular [compartment marker] of thylakoid membraneSpecies of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions. In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.Rabbit anti-PsbA antibody can detect more than one band of PsbA protein, e.g. precursor and mature protein as compare to the hen anti-PsbA antibodies AS01 016.This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel.The antibody will bind to cross-linked proteins: D1/D2, D1/cyt b559, D1/CP43.

Background: The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: ATCG00020

Category: Thylakoid membrane marker

Research Area: Plant Biology, Photosynthesis