PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-protein) | AS06 142-33

PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-protein) | AS06 142-33 | Gentaur UK, US & Europe Distribution

Immunogen: Native purified 33 kDa protein from Spinacia oleracea UniProt: P12359

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Total IgG. Protein G purified in PBS pH 7.4.

Format: Lyophilized

Tested Application: Immunoprecipitation (IP), Western blot (WB)

Related Products: AS14 2824 | Anti-PsbO1 | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodies AS14 2825 | Anti-PsbO2 | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodiesAS05 092 | Anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodiesAS06 167 | Anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodiesAS08 305 | Anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodiesAS06 142-16 | Anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodiesPlant protein extraction buffer Secondary antibodies

Recommended Dilutions: 1 : 2000-1 : 5000 (WB)

Molecular weight: 35 | 33 kDa

Confirmed Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Chlorella sp. DT, Chlorella vulgaris, Echinochloa crus-galli, Festuca arundinacea cv. Kord, Haematococcus pluvialis, Hordeum spontaneum, Hordeum vulgare, Nicotiana benthamiana, Oryza sativa, Panicum miliaceum, Picea abies, Pinus banksiana, Pisum sativum, Spinacia oleracea, Solanum tuberosum cultivar Taedong Valley, Synechococcus sp. PCC7002, sp. PCC 7942 and sp. PCC6803, Thellungiella salsuginea, Triticum aestivum, Zea mays

Predicted Reactivity: Nicotiana tabacum, Solanum lycopersicum, Populus trichocarpa, Zosteria marinaSpecies of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007) .

Background: PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: PSII (Photosystem II)

Research Area: Plant Biology, Photosynthesis