PUF60 polyclonal Antibody | BS8373

PUF60 polyclonal Antibody | BS8373 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB,IHC,IF

Application Range: WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Background: Activation of FUSE, the far-upstream element, is required for the proper expression of the mammalian gene c-myc. The binding of FBP (FUSE-binding protein) to FUSE is necessary for c-myc expression. The FBP interacting repressor, FIR, binds to the central DNA-binding domain of FBP and can serve as an overriding negative regulator of c-myc promoter activity. FIR interacts with the TFIIH complex, which is a multifunctional, multisubunit RNA polymerase II transcription factor that interacts with several DNA-binding transactivators. FIR blocks activator-dependent, but not basal transcription through TFIIH. FIR shares identity with seven in absentia (siah) binding protein 1. FIR is expressed in spleen, thymus, prostate, small intestine, colon, and peripheral blood leukocytes, and with relatively higher levels of expression in testis and ovary .

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PUF60 polyclonal Antibody detects endogenous levels of PUF60 protein.

Molecular Weight: ~ 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Poly (U) -binding-splicing factor PUF60; 60 kDa poly (U) -binding-splicing factor; FUSE-binding protein-interacting repressor; FBP-interacting repressor; Ro-binding protein 1; RoBP1; Siah-binding protein 1; Siah-BP1; PUF60; FIR; ROBPI; SIAHBP1

Immunogen: Recombinant full length Human PUF60.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: