PUMA Antibody | 3041

PUMA Antibody | 3041 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Rat: (100%)

Immunogen: Anti-PUMA antibody (3041) was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus human PUMA isoform 1.
The immunogen is located within the last 50 amino acids of PUMA.

Research Area: Apoptosis, Neuroscience, Autophagy, Cancer

Tested Application: E, WB, ICC, IF

Application: WB: 1-4 μg/mL; IF: 2 μg/mL; ICC: 1 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: A lower band at approximately 16 kDa was detected in Daudi and K562 cells, which may represent the PUMA isoform 2.

Positive Control 1: Cat. No. 1210 - 293 Cell Lysate

Positive Control 2: Cat. No. 1211 - HepG2 Cell Lysate

Positive Control 3: Cat. No. 1220 - SK-N-SH Cell Lysate

Positive Control 4: Cat. No. 1204 - K562 Cell Lysate

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 23 kDa

Validation:

Independent Antibody Validation (Figure 2) shows similar PUMA expression profile in both human and mouse cell lines detected by two independent anti-PUMA antibodies that recognize different epitopes, 3041 against C-terminus and 3043 against the N-terminus.  PUMA proteins are detected in most of the cell lines with different expression levels by the two independent antibodies.  

siRNA Knockdown Validation (Figure 3) : Anti-PUMA antibody (3041) specificity was further verified by PUMA specific siRNA knockdown. PUMA signal in HeLa cells transfected with PUMA siRNAs was much weaker in comparison with that in HeLa cells transfected with control siRNAs.

Isoform: Human PUMA has 4 isoforms, including isoform 1 (193aa, 21kD) , isoform 2 (131aa, 14kD) , isoform 3 (101aa, 10kD) and isofrom 4 (261aa, 27kD) . This antibody detects human isoform 1&2, mouse and rat PUMA (193aa, 21kD for both of them) .

Purification: PUMA Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PUMA Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: PUMA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: PUMA Antibody: JFY1, PUMA, JFY-1, Bcl-2-binding component 3

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: PUMA Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53 (1) . The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (2) . PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.