PUMA (R169) polyclonal Antibody | BS2922

PUMA (R169) polyclonal Antibody | BS2922 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB

Application Range: WB: 1:500~1:1000

Background: PUMA (Bcl-2 binding component 3, JFY1, PUMA/JFY1) is a mitochondrial pro-apoptotic Bcl-2 homology domain (BH3) -only protein that induces rapid apoptosis through a Bax- and mitochondria-dependent pathway. The PUMA gene encodes four proteins originating from different splice variants of the same transcript: PUMAα, β, γ and δ. Both PUMAα and PUMAβ contain a BH3 domain, while PUMAγ and PUMAδ lack this domain. The BH3 domain is essential for binding of PUMAα and PUMAβ to Bcl-2 or Bcl-xL. PUMA is an initiator of γ-radiation apoptosis and glucocorticoid-induced apoptosis in lymphoid cells in vivo. Bcl-2 family members generally regulate apoptosis and transmit death signals to mitochondria. Members of this family include both pro- and anti-apoptotic proteins that share homologous sequences known as Bcl-2 homology domains (BH1-4) . The BH3 proteins, BID, NOXA, PUMA, NBK, Bim and Bad, are all pro-apoptotic and share sequence homology within the amphipathic α-helical BH3 region.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PUMA (R169) polyclonal Antibody detects endogenous levels of PUMA protein.

Molecular Weight: ~ 15, 20, 28 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Bcl-2-binding component 3; JFY-1; p53 up-regulated modulator of apoptosis; BBC3; PUMA

Immunogen: Synthetic peptide, corresponding to amino acids 132-183 of Human PUMA.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: