PUS1 Antibody | 28-640

PUS1 Antibody | 28-640 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Mouse, Rat

Homology: N/A

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse PUS1.

Research Area: Transcription

Tested Application: E, WB

Application: PUS1 antibody can be used for detection of PUS1 by ELISA at 1:312500. PUS1 antibody can be used for detection of PUS1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.

Specificiy: N/A

Positive Control 1: SP2/0 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 43 kDa

Validation: N/A

Isoform: N/A

Purification: Antibody is purified by peptide affinity chromatography method.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Concentration: batch dependent

Storage Condition: For short periods of storage (days) store at 4˚C. For longer periods of storage, store PUS1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Alternate Name: PUS1, MPUS1, mPus1p, A730013B20Rik

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Pus1 converts specific uridines to PSI in a number of tRNA substrates. Pus1 acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Pus1 is involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA.