Description
PYCARD Antibody | 45-129 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human
Homology: N/A
Immunogen: The immunogen for this antibody is: C-RESQSYLVEDLERS
Research Area: Apoptosis
Tested Application: E, WB, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 19kDa band observed in lysates of cell line HeLa (calculated MW of 20.0kDa according to NP_660183.1) . Recommended concentration: 0.2-0.6ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Liver shows staining of sinusoidal lining cells. Recommended concentration: 4-6ug/ml.
Specificiy: This antibody is expected to recognise both reported isoforms (NP_037390.2 and NP_660183.1) .
Positive Control 1: Cat. No. 1215 - U937 Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: PYCARD, ASC, TMS1, PYD and CARD domain containing, CARD5, MGC10332, TMS, apoptosis-associated speck-like protein containing a CARD, caspase recruitment domain protein 5, target of methylation-induced silencing-1, TMS-1
User Note: N/A
BACKGROUND: N/A