Pyridoxal 5’-phosphate (Vit B6) Fluorometric Assay Kit - K2009

K2009 | Pyridoxal 5’-phosphate (Vit B6) Fluorometric Assay Kit DataSheet

Pyridoxal 5’-phosphate (PLP), the active form of Vitamin B6 is a cofactor in a variety of metabolic reactions including transamination, decarboxylation, racemization, etc. Vitamin B6 comprises of pyridoxine, pyridoxamine and pyridoxal, which during metabolism is converted to the enzymatically active form namely PLP. Vitamin B6 is necessary for the synthesis of neurotransmitters, hemoglobin in red blood cells, fat metabolism etc. Vitamin B6 deficiency can lead to nervous disorders, peripheral neuropathy, anemia, seizures etc. ’s Pyridoxal 5’-phosphate (Vit B6) Fluorometric Assay Kit provides a quick, specific and easy method for measuring PLP concentrations in a wide variety of samples. In this assay, PLP reacts with a PLP-dependent enzyme thereby converting the substrate into an intermediate, which will further react with a probe to produce a strong fluorometric signal (Ex/Em = 535/587 nm). The kit is simple, easy to use, sensitive and high-throughput adaptable. It can detect as low as 0.2 pmol/well of PLP in biological samples.

Alternate Name : Vit B6 Fluorometric Kit, B6 Fluorometric Assay, Vit B6 Fluorometric Assay, B6 Fluorometric Kit, B6 Fluorometric, Vit B6 Fluorometric Assay Kit, Vitamin B6 Fluorometric Kit, PLP, Vitamin B6 Assay, B6 Fluorometric Assay Kit, Vit B6 Assay Kit, Pyridoxal 5’-phosphate, Vitamin B6 Fluorometric Assay Kit, Pyridoxal 5’-phosphate (Vit B6) Fluorometric Assay Kit, Pyridoxal 5’-phosphate (Vit B6) Fluorometric Assay, Vitamin B6 Fluorometric Assay, Pyridoxal 5’-phosphate Fluorometric Assay Kit, Vitamin B6 Assay Kit, Pyridoxal phosphate, Vitamin B6, Pyridoxal 5’-phosphate (Vit B6) Assay Kit

Tag Line : for measuring PLP concentrations

Summary : Pyridoxal 5’-phosphate (PLP), the active form of Vitamin B6 is a cofactor in a variety of metabolic reactions including transamination, decarboxylation, racemization, etc. Vitamin B6 comprises of pyridoxine, pyridoxamine and pyridoxal, which during metabolism is converted to the enzymatically active form namely PLP. Vitamin B6 is necessary for the synthesis of neurotransmitters, hemoglobin in red blood cells, fat metabolism etc. Vitamin B6 deficiency can lead to nervous disorders, peripheral neuropathy, anemia, seizures etc. ’s Pyridoxal 5’-phosphate (Vit B6) Fluorometric Assay Kit provides a quick, specific and easy method for measuring PLP concentrations in a wide variety of samples. In this assay, PLP reacts with a PLP-dependent enzyme thereby converting the substrate into an intermediate, which will further react with a probe to produce a strong fluorometric signal (Ex/Em = 535/587 nm). The kit is simple, easy to use, sensitive and high-throughput adaptable. It can detect as low as 0.2 pmol/well of PLP in biological samples.

Detection Method : Fluorescence detection (Ex/Em = 535/587 nm)

Sample Type : Biological fluids: serum, juices, etc., Mammalian tissue

Species Reactivity : All

Applications : Analysis of Vitamin B6 in beverage and effects of Vitamin B6 intake on serum, Measurement of PLP in biological samples and beverages

Features and Benefits : Specific, Easy method for measuring PLP concentrations in a wide variety of samples., It can detect as low as 0.2 pmol/well of PLP in biological samples, Quick