Pyruvate Dehydrogenase (C-term) monoclonal Antibody | MB0082

Pyruvate Dehydrogenase (C-term) monoclonal Antibody | MB0082 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human,Mouse

Application: WB ICC

Application Range: WB: 1:1000 ICC: 1:100~200

Background: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO (2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) . The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This Antibody detects endogenous levels of Pyruvate Dehydrogenase (C-terminus) and does not cross-react with related proteins

Molecular Weight: Predicted band size:43KDa Observed band size:43KDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHE1-A type I; PDHA1; PHE1A

Immunogen: Synthetic peptide, corresponding to the C-terminus of Human Pyruvate Dehydrogenase.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: