QSOX1 Antibody | 8171

QSOX1 Antibody | 8171 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Rat: (76%)

Immunogen: QSOX1 antibody was raised against a 17 amino acid peptide near the center of human QSOX1.
The immunogen is located within amino acids 360 - 410 of QSOX1.

Research Area: Cell Cycle, Cancer, Apoptosis

Tested Application: E, WB, IHC-P, IF

Application: QSOX1 antibody can be used for detection of QSOX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: QSOX1 antibody is human and mouse reactive. QSOX1 antibody is predicted to not cross-react with QSOX2.

Positive Control 1: Cat. No. 1287 - EL4 Cell Lysate

Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 65, 82 kDa
Observed: 66 kDa

Validation: N/A

Isoform: N/A

Purification: QSOX1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: QSOX1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: QSOX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Quiescin Q6 sulfhydryl oxidase 1, Sulfhydryl oxidase 1, Q6, QSCN6

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The QSOX1 gene, also known as Quiescin Q6, is a fusion of two ancient genes: thioredoxin and ERV1. Its expression is induced as fibroblasts begin to exit the proliferative cycle and enter quiescence, suggesting that this gene plays an important role in growth regulation (1) . The QSOX1 protein oxidizes sulfhydryl groups to form disulfide bonds in proteins. QSOX1 expression is induced by hypoxia (2) and appears to protect cells against oxidative stress-induced apoptosis (3) .