65

QuantiChrom™ Alcohol dehydrogenase Assay Kit

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SKU:
65-DADH-100-GEN
Availability:
In Stock
NULL770.80

Description

QuantiChrom™ Alcohol dehydrogenase Assay Kit

 

Application: For quantitative determination of alcohol dehydrogenase activity and evaluation of drug effects on its metabolism.

Key Features:

  • Fast and sensitive. Linear detection range (20 μL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction.
  • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method: OD565nm

Samples: Biological (e.g. plasma, serum, urine, tissue and culture media)

Species: All

Size: 100 tests

Detection Limit: 0.4 U/L

Shelf Life: 6 months

More Details:

ALCOHOL DEHYDROGENASE (ADH) is an oxidoreductase which catalyzes the interconversion of alcohols and aldehydes or ketones. ADH is important in humans and other organisms for the break down of alcohols which may otherwise be toxic.

In yeast and some bacteria, ADHs catalyze the opposite reaction and produce alcohol as part of fermentation. Gentaur Genprice non-radioactive, colorimetric ADH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

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Additional Information

Application:
For quantitative determination of alcohol dehydrogenase activity
Method:
OD565nm
Samples:
serum, plasma, cell, tissue, agriculture etc
Species:
all
Procedure:
30 min
Size:
100 Tests
Detection limit:
0.1 U/L
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